The paper-based toehold switch sensor is convenient and efficient and can perform pathogen detection without site limitations, which plays a great role in disease detection, prevention, and control. However, a paper-based toehold switch sensor using an isothermal amplification technique has the disadvantages of long reaction time and relatively low detection limit. Considering that rRT-PCR is a commonly used laboratory method for RNA virus detection, CD BioSciences introduced reverse transcription loop-mediated amplification (RT-LAMP) into the biosensor to provide you with a fast and efficient paper-based toehold switch sensor with the modified RT-LAMP for specific targets.
As in the case of conventional paper-based cell-free toehold switch sensor development, in this development service, we also need to design toehold switch RNA specific to your target to be tested and an isothermal amplification technique to amplify the signal. The difference is that we replaced the NASBA amplification method with the RT-LAMP amplification method. RT-LAMP has the advantage of rapid amplification; however, the amplification product of this method is DNA, which cannot react directly with the toehold switch RNA. Therefore, our challenge is to improve RT-LAMP so that its amplification product is converted to RNA.
Our paper-based toehold switch sensor with the modified RT-LAMP development service has the following 4 main steps.
1. Toehold switch RNA design. Firstly, find the target-specific DNA sequence and then design the toehold switch RNA based on this sequence. We will optimize the toehold switch to ensure that it can be successfully triggered by the trigger RNA.
2. Preparation of the paper-based carrier. We will perform a series of operations such as modification and sterilization of the paper-based carrier to ensure the successful development of the sensor.
3. Preparation of the cell-free reaction mixture. In this step, we will mix the enzymes, solutions, etc. needed for the cell-free reaction in the optimal ratio and load them onto the paper-based platform.
4. Coupling of RT-LAMP with toehold switch reaction. In this step, the primers for RT-LAMP will be designed first. Then, we will convert the RT-LAMP isothermal amplified products into RNA for the next cell-free reaction.
CD BioSciences is dedicated to toehold switch related research. With our strong technology platform and professional researchers, we are fully capable of providing you with a paper-based toehold switch sensor with the modified RT-LAMP development service to meet your needs. If you are interested in this service, please feel free to contact us and we will be at your service 24 hours a day.