Toehold Switch Design Service

Toehold Switch Design Service

"Toehold Switch" is a specifically engineered RNA molecule that has the ability to precisely manipulate genomic sequences. The genome is the genetic blueprint that determines the characteristics of an organism, so the ability to manipulate the toehold switch can lead to tangible changes in an organism, providing important insights into the cellular environment and potential diseases, as well as providing excellent tools for gene therapy of diseases. However, in practical applications, the toehold switch is difficult to perform to the desired function. Therefore, CD BioSciences uses deep machine learning models to improve this situation.

Our Services

At CD BioSciences, we are able to use our technology platform to design the most appropriate toehold switch for the trigger RNA sequence. Toehold switch design involves a number of factors that need to be carefully considered. First, we need to understand the various dynamic molecular mechanisms involved in the cell and, more importantly, we need to consider the base complementarity between the toehold switch and the sequence of the trigger RNA.

• When determining the trigger RNA sequence, make sure that the sequence chosen is unique to that trigger RNA to avoid off-target effects.
• Ensure that the ribosome binding site and the start codon in the toehold switch should be separated and should not contain a stop codon to avoid affecting the translation of downstream genes.
• Ensure that the hairpin structure of the toehold switch is stable enough to avoid that no trigger RNA is detected but the hairpin structure opens automatically, allowing downstream genes to be translated.
• Ensure that the double strand of the toehold switch bound to the trigger RNA has an energetic advantage so that the hairpin structure of the toehold switch can be activated and unfolded smoothly after the interaction.

Our Advantages

• We are able to provide one-stop service for toehold switch design.
• Our experienced research team is familiar with various genetic manipulation elements.
• Our technology platform allows us to design toehold switches with specified promoters, ribosome binding sites, and lengths according to the parameters provided by our customers.

We Are Ready to Provide

CD BioSciences can provide you with a toehold switch design service using an advanced technology platform. We guarantee that this toehold switch design service will fully meet your needs and comply with the relevant laws and regulations. We are available to you 24 hours a day, so please feel free to contact us if you need us.

References

1. To, A.C.; et al. A comprehensive web tool for toehold switch design. Bioinformatics. 2018, 34(16): 2862-2864.
2. Angenent-Mari, N.M.; et al. A deep learning approach to programmable RNA switches. Nat Commun. 2020, 11(1): 5057.
3. Valeri, J.A.; et al. Sequence-to-function deep learning frameworks for engineered riboregulators.Nat Commun. 2020, 11(1): 5058.